Crystal structure of the zinc-, cobalt-, and iron-containing adenylate kinase from Desulfovibrio gigas: a novel metal-containing adenylate kinase from Gram-negative bacteria
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چکیده
منابع مشابه
Structural stability of adenylate kinase from the sulfate-reducing bacteria Desulfovibrio gigas.
A novel adenylate kinase (AK) has recently been purified from Desulfovibrio gigas and characterized as a Co(2+)/Zn(2+)-containing enzyme: this is an unusual characteristic for AKs from Gram-negative bacteria, in which these enzymes are normally devoid of metals. Here, we studied the conformational stability of holo- and apo-AK as a function of temperature by differential scanning calorimetry (D...
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Adenylate kinases (ADK) are ubiquitous enzymes that contribute to the homeostasis of adeninenucleotides in living cells. In this study, the cloning of a cDNA encoding an adenylate kinase from the filariaOnchocerca volvulus has been described. Using PCR technique, a 281 bp cDNA fragment encoding part ofan adenylate kinase was isolated from an O. volvulus cDNA library. Use of this fragment as a p...
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Adenylate kinases (AKs) play important roles in nucleotide metabolism in all organisms and in cellular energetics by means of phosphotransfer networks in eukaryotes. The crystal structure of a human AK named AK6 was determined by in-house sulfur single-wavelength anomalous dispersion phasing methods and refined to 2.0-A resolution with a free R factor of 21.8%. Sequence analyses revealed that h...
متن کاملmolecular cloning of adenylate kinase from the human filarial parasite onchocerca volvulus
adenylate kinases (adk) are ubiquitous enzymes that contribute to the homeostasis of adeninenucleotides in living cells. in this study, the cloning of a cdna encoding an adenylate kinase from the filariaonchocerca volvulus has been described. using pcr technique, a 281 bp cdna fragment encoding part ofan adenylate kinase was isolated from an o. volvulus cdna library. use of this fragment as a p...
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The 5'-AMP (adenosine monophosphate)-activated protein kinase (AMPK) coordinates metabolic function with energy availability by responding to changes in intracellular ATP (adenosine triphosphate) and AMP concentrations. Here, we report crystal structures at 2.9 and 2.6 A resolution for ATP- and AMP-bound forms of a core alphabetagamma adenylate-binding domain from the fission yeast AMPK homolog...
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ژورنال
عنوان ژورنال: JBIC Journal of Biological Inorganic Chemistry
سال: 2010
ISSN: 0949-8257,1432-1327
DOI: 10.1007/s00775-010-0700-8